Dr Tracey Madgett
Profiles

Dr Tracey Madgett

Lecturer in Molecular Biology

School of Biomedical Sciences (Faculty of Health)

Biography

Biography

Programme Lead for BSc (Hons) Biomedical Science 
UoP Node Lead for Biomedical Science for the TNE partnership with NSBM Green University, Sri Lanka.
Co-Chair of the Faculty of Health Research Ethics and Integrity Taught Masters Sub-Committee.
Lead for the Blood Diagnostics Research Group 

Qualifications

B.A. Hons. Natural Sciences - Cambridge University (Newnham College), 1996
M.A. - Cambridge University, 2000
Ph.D. - The regulation of cyclooxygenase-2 in an in vitro model of colorectal tumorigenesis and mechanisms of action of potential chemopreventive agents. Bristol University, 2000
Post-Graduate Certificate in Academic Practice (PGCAP) - Plymouth University, 2015

Background
Prior to joining Plymouth University in 2010, Tracey completed post-doctoral studies at Bath University (2000-2003) and the University of the West of England, Bristol (2003-2010). She became proficient in a variety of laboratory techniques and alongside her own research was able to support undergraduate students during their final year projects. Cancer research made way for blood group genotyping research and then non-invasive prenatal diagnosis research (with the group of Prof Neil Avent).
On arrival in Plymouth in 2010, Tracey managed the day-to-day running of the laboratory of Prof Neil Avent (including supervision of Ph.D. students and undergraduates). In 2011, she was heavily involved in the design of the refurbishment of the laboratories for the Plymouth University Systems Biology Centre (genomics and proteomics facilities) and became the Director of the Centre. This led to key involvement in the design of the Derriford Research Facility, which opened in September 2017.  Following Neil Avent's retirement in March 2018, Tracey became the group lead for the Blood Diagnostics Research Group, based at the Derriford Research Facility. 
Following a number of years as co-lead for the School of Biomedical Sciences' Final Year Personal Research Project module, Tracey became Programme Lead for BSc (Hons) Biomedical Science in May 2021.  Tracey's role has moved to have a more teaching-intensive focus in recent years, with teaching on modules from Foundation through undergraduate to Masters level. 
Tracey is UoP Node Lead for Biomedical Science for the TNE partnership with NSBM Green University, Sri Lanka.

Professional membership

Fellow, Higher Education Academy

Key publications

Sillence KA, Roberts LA, Hollands HJ, Thompson HP, Kiernan M, Madgett TE, Ross Welch C & Avent ND (2015) 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 ,
View all publications
Teaching

Teaching

Teaching interests

Main teaching areas: molecular biology, non-invasive prenatal testing, blood groups and genomics/proteomics technologies.
Personal Tutor for Biomedical Sciences (BHCS1011, BHCS2027 and BHCS3031)
Foundation year: FMD001 (Molecules to Cells), FMD002 (Introduction to Human Physiology; Module lead) and BHCS002 (Current Developments in Human Biology and Biomedical Sciences)
First year: BHCS1005 (Human Disease)
Second year: BHCS2002 (Evidence-based practice in Biomedical Science), BHCS2003 (Genetic Continuity and Diversity), BHCS2005 (Clinical Haematology and Biochemistry) and BHCS2019 (Methods in Human Biosciences)
Third year: BHCS3001 (Personal Research Project), BHCS3004 (Specialist Biochemistry and Screening), BHCS3005 (Transplantation and Transfusion Science), BHCS3008 (Current Developments in Biomedical Science; Module Lead) and BHCS3014 (Reproductive Science) 
Masters level: BIOM5001 (Molecular Biology: Genomics, Transcriptomics and Proteomics), BIOM5005 (Project Design and Development) and BIOM5006 (Research Project).  Also MCR702 Applied Quantitative Research Methods in School of Health Professions.
Research

Research

Research interests

Molecular diagnostics in the fields of transfusion medicine and non-invasive prenatal testing, primarily blood group genotyping (with a particular interest in RHDgenetics), free fetal DNA enrichment methods and finding new protein biomarkers for Down Syndrome in maternal plasma. Research focus involves using technologies such as next generation sequencing, digital PCR and mass spectrometry/proteomics.
Current interests include investigating the role of CD47 and determining the CD47 binding partners in colorectal cancer cell lines, investigating the anti-proliferative activity of natural extracts in colorectal cancer cell lines and cryopreservation of red blood cells.
Current students - DoS for ResM student (Evaluation of novel cryoprotectants for the long term storage of red blood cell units)
UoP Research Group Membership
Plymouth Institute of Health and Care Research (PIHR)

Research groups

Research degrees awarded to supervised students

Dr Raed Garout - Molecular Pathology: Potential Biomarkers for the Detection of Down Syndrome Pregnancies (Oct 2022)
Dr Wajnat Tounsi - High Throughput Sequencing Technologies in Blood Group Genotyping and Applications in Transfusion Medicine: Establishment of Rh Genes Reference Allele Sequences (June 2021)
Dr Mashael Alkhanbashi - Biochemistry: Production of recombinant Rh blood group antigens for detection of alloimmunisation (December 2019)
Dr Ibrahim Halawani - Role of CD47 in Red Cell Apoptosis (Eryptosis) (December 2019)
Dr Malik Altayar - Next Generation Sequencing-Based Genotyping of Human Blood Groups: FY, JK and ABO Genes (March 2017)
Dr Kelly Sillence - Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques (May 2016)
Dr Amr Halawani - The Future of Next-generation Sequencing for Blood Group Genotyping and its Implications in Transfusion Medicine (March 2016)

Grants & contracts

Northcott Devon Medical Foundation (2019-date) (£10K)
Publications

Publications

Key publications

Sillence KA, Roberts LA, Hollands HJ, Thompson HP, Kiernan M, Madgett TE, Ross Welch C & Avent ND (2015) 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 ,

Key publications are highlighted

Journals
Articles
Madgett TE, Tounsi WA, Halawani AJ & Avent ND (2023) 'RHD molecular analysis—from discovery to next generation sequencing' Annals of Blood ,
Madgett TE (2022) 'First Trimester Noninvasive Prenatal Diagnosis of Maternally Inherited Beta-Thalassemia Mutations' Clinical Chemistry ,
Tounsi WA, Lenis VP, Tammi SM, Sainio S, Haimila K, Avent ND & Madgett TE (2022) 'Rh blood group D antigen genotyping using a portable nanopore based sequencing device: proof of principle' Clinical Chemistry ,
Tammi SM, Tounsi WA, Sainio S, Kiernan M, Avent ND, Madgett TE & Haimila K (2020) 'Next-generation sequencing of 35 RHD variants in 16 253 serologically D− pregnant women in the Finnish population' Blood Advances 4, (20) 4994-5001 ,
(2019) 'Tounsi WA, Madgett TE, Avent ND. Complete RHD next-generation sequencing: establishment of reference RHD alleles. Blood Adv. 2018;2(20):2713-2723' Blood Advances 3, (2) 120-120 ,
Tounsi WA, Madgett TE & Avent ND (2018) 'Complete RHD next-generation sequencing: establishment of reference RHD alleles' Blood Advances 2, (20) 2713-2723 ,
Sillence KA, Halawani AJ, Tounsi WA, Clarke KA, Kiernan M, Madgett TE & Avent ND (2017) 'Rapid RHD Zygosity Determination Using Digital PCR' Clinical Chemistry ,
Sillence KA, Roberts LA, Hollands HJ, Thompson HP, Kiernan M, Madgett TE, Ross Welch C & Avent ND (2015) 'Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR' Clinical Chemistry 61, (11) 1399-1407 ,
Li B, Pan G, Avent ND, Lowry RB, Madgett TE & Waines PL (2015) 'Graphene electrode modified with electrochemically reduced graphene oxide for label-free DNA detection' Biosensors and Bioelectronics 72, 313-319 ,
Haer-Wigman L, Veldhuisen B, Jonkers R, Lodén M, Madgett TE, Avent ND, de Haas M & van der Schoot CE (2013) 'RHD and RHCE variant and zygosity genotyping via multiplex ligation-dependent probe amplification' Transfusion 53, (7) 1559-1574 ,
Sillence KA, Madgett TE, Roberts LA, Overton TG & Avent ND (2013) 'Non-invasive screening tools for Down's Syndome: A review' Diagnostics 3, (2) 291-314 ,
Avent ND, Webb A, Madgett TE, Miran T, Sillence K, Kaushik N & Kiernan M (2012) 'Non Invasive Prenatal Diagnosis of Aneuploidy: Next Generation Sequencing or Fetal DNA Enrichment?' Balkan Journal of Medical Genetics 15, (Supplement) 17-26 ,
Heywood W, Wang D, Madgett TE, Avent ND, Eaton S, Chitty LS & Mills K (2012) 'The development of a peptide SRM-based tandem mass spectrometry assay for prenatal screening of Down syndrome' J Proteomics 75, (11) 3248-3257 ,
Heywood W, Mills K, Wang D, Hogg J, Madgett TE, Avent ND & Chitty LS (2012) 'Identification of new biomarkers for Down's syndrome in maternal plasma' J Proteomics 75, (9) 2621-2628 ,
Heywood WE, Madgett TE, Wang D, Wallington A, Hogg J, Mills K & Avent ND (2011) '2D DIGE analysis of maternal plasma for potential biomarkers of Down Syndrome' Proteome Sci 9, ,
Wigman L, Veldhuisen B, Soussan AA, Avent ND, Madgett TE, Maddocks DG, de Haas M & van der Schoot CE (2010) 'DEVELOPMENT OF A MULTIPLEX LIGAND-DEPENDENT PROBE AMPLIFICATION (MLPA) ASSAY TO DETERMINE VARIANTS IN THE RHD AND RHCE GENE' VOX SANGUINIS 99, 506-506
Jeremy KP, Plummer ZE, Head DJ, Madgett TE, Sanders KL, Wallington A, Storry JR, Gilsanz F, Delaunay J & Avent ND (2009) '4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins' Haematologica 94, (10) 1354-1361 ,
Avent ND, Madgett TE, Maddocks DG & Soothill PW (2009) 'Cell-free fetal DNA in the maternal serum and plasma: current and evolving applications' Curr Opin Obstet Gynecol 21, (2) 175-179 ,
Maddocks DG, Alberry MS, Attilakos G, Madgett TE, Choi K, Soothill PW & Avent ND (2009) 'The SAFE project: towards non-invasive prenatal diagnosis' Biochem Soc Trans 37, (Pt 2) 460-465 ,
Avent ND, Martinez A, Flegel WA, Olsson ML, Scott ML, Nogués N, Písacka M, Daniels GL, Muñiz-Diaz E & Madgett TE (2009) 'The Bloodgen Project of the European Union, 2003-2009' Transfus Med Hemother 36, (3) 162-167 ,
Avent ND, Plummer ZE, Madgett TE, Maddocks DG & Soothill PW (2008) 'Post-genomics studies and their application to non-invasive prenatal diagnosis' Semin Fetal Neonatal Med 13, (2) 91-98 ,
Avent ND, Martinez A, Flegel WA, Olsson ML, Scott ML, Nogués N, Písacka M, Daniels G, van der Schoot E & Muñiz-Diaz E (2007) 'The BloodGen project: toward mass-scale comprehensive genotyping of blood donors in the European Union and beyond' Transfusion 47, (1 Suppl) 40S-46S ,
Avent ND, Madgett TE, Lee ZE, Head DJ, Maddocks DG & Skinner LH (2006) 'Molecular biology of Rh proteins and relevance to molecular medicine' Expert Rev Mol Med 8, (13) 1-20 ,
Fox BC, Crew TE & Welham MJ (2005) 'Phosphoinositide 3-kinases can act independently of p27Kip1 to regulate optimal IL-3-dependent cell cycle progression and proliferation' Cell Signal 17, (4) 473-487 ,
Charalambous M, Smith FM, Bennett WR, Crew TE, Mackenzie F & Ward A (2003) 'Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism' Proc Natl Acad Sci U S A 100, (14) 8292-8297 ,
Bennett WR, Crew TE, Slack JMW & Ward A (2003) 'Structural-proliferative units and organ growth: effects of insulin-like growth factor 2 on the growth of colon and skin' Development 130, (6) 1079-1088 ,
Elder DJ, Halton DE, Crew TE & Paraskeva C (2000) 'Apoptosis induction and cyclooxygenase-2 regulation in human colorectal adenoma and carcinoma cell lines by the cyclooxygenase-2-selective non-steroidal anti-inflammatory drug NS-398' Int J Cancer 86, (4) 553-560 ,
Crew TE, Elder DJ & Paraskeva C (2000) 'A cyclooxygenase-2 (COX-2) selective non-steroidal anti-inflammatory drug enhances the growth inhibitory effect of butyrate in colorectal carcinoma cells expressing COX-2 protein: regulation of COX-2 by butyrate' Carcinogenesis 21, (1) 69-77 ,
Conference Papers
Avent ND, Madgett TE, Halawani AJ, Altayar MA, Kiernan M, Reynolds AJ & Li X (2015) 'Next‐generation sequencing: academic overkill or high‐resolution routine blood group genotyping?' Wiley 250-256 ,
Madgett TE, Maddocks DG, Nygren A, Choi K & Avent ND (2005) 'Rapid molecular genotyping for RHD using multiplex ligation-dependent probe amplification technology' 129A-129A
Smith F, Charalambous M, Bennett W, Crew T, Mackenzie F & Ward A (2004) 'Disruption of the imprinted <i>Grb10</i> gene in mice leads to disproportionate overgrowth with effects on metabolism' 117-118
Charalambous M, Bennett WR, Smith F, Crew TE, Mackenzie F & Ward A (2003) 'Growth regulation by <i>Grb10</i>' 236-236
Paraskeva C, Hague A, Crew T & Elder DJE (1999) 'Chemoprevention of colon cancer: diet NSAIDs and apoptosis' 568-568
Other Publications
Madgett TE (2008) Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader.
Personal

Personal

Other academic activities

External examiner for UWE, Bristol (July 2021 - September 2024)